Microfluidics integrated n-type organic electrochemical transistor for metabolite sensing

The organic electrochemical transistor (OECT) can translate biochemical binding events between a recognition unit and its analyte into an electrical signal. We present OECT comprising n-type (electron transporting) conjugated polymer-based channel lateral gate electrode functionalized with the enzyme, glucose oxidase. device is integrated microfluidic system for real-time monitoring in flow-through manner. polymer has direct communication oxidase, allowing detection while surpassing hydrogen peroxide production. microfluidic-integrated shows superior features compared to microfluidic-free counterpart, including higher current transconductance values as well improved signal-to-noise (SNR) ratios, which enhances sensor sensitivity limit. Thanks low noise endowed by microfluidics, changes upon metabolite could be resolved, revealing that relative drain currents are similar, output SNR. This first demonstration of integration accumulation mode enzymatic detection. design provides new insights mechanisms leading high sensitivities, crucial development portable autonomous lab-on-a-chip technologies.